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Top rated chemical buffers

Here are some top-rated chemical buffers, widely used in various laboratory and industrial applications:

1. HEPES (4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid): A zwitterionic buffer with a pKa of 7.5, commonly used in biological and biochemical applications.
2. PBS (Phosphate-Buffered Saline): A mixture of sodium phosphate and sodium chloride, commonly used in molecular biology, cell culture, and biochemistry.
3. Tris (Tris(hydroxymethyl)aminomethane)): A basic buffer with a pKa of 8.3, widely used in biochemistry, molecular biology, and biotechnology applications.
4. MOPS (3-(N-Morpholino)propanesulfonic acid): A zwitterionic buffer with a pKa of 7.2, commonly used in biochemistry, molecular biology, and enzymology.
5. Bicine (N,N-Bis(2-hydroxyethyl)glycine): A zwitterionic buffer with a pKa of 8.3, commonly used in biochemistry, molecular biology, and biotechnology applications.
6. TES (N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid): A zwitterionic buffer with a pKa of 7.5, commonly used in biochemistry, molecular biology, and biotechnology applications.
7. Pipes (Piperazine-N,N'-bis(2-ethanesulfonic acid)): A zwitterionic buffer with a pKa of 6.8, commonly used in biochemistry, molecular biology, and biotechnology applications.
8. HCl/KCl (Hydrochloric acid/Potassium chloride): A_buffer system commonly used in analytical chemistry, pharmaceutical, and biomedical applications.
9. Na2HPO4/NaH2PO4 (Sodium phosphate): A buffer system commonly used in biochemistry, molecular biology, and biotechnology applications.
10. CAPS (3-Cyclohexylamino-1-propanesulfonic acid): A zwitterionic buffer with a pKa of 10.4, commonly used in biochemistry, molecular biology, and biotechnology applications.

These buffers are widely available from various manufacturers, including:

• Sigma-Aldrich (now part of Merck)
• Thermo Fisher Scientific
• Invitrogen (now part of Thermo Fisher Scientific)
• Alfa Aesar
• MP Biomedicals
• Calbiochem (now part of Merck)

When selecting a chemical buffer, consider the following factors:

• pH range: Choose a buffer with a pKa close to the desired pH.
• Buffer capacity: Select a buffer with a suitable buffer capacity for your application.
• Interference: Consider potential interference from the buffer components with your experimental system.
• Cost: Evaluate the cost of the buffer and the availability of the components.
• Stability: Choose a buffer with good chemical stability and a long shelf life.