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Top rated chemical buffers
Here are some top-rated chemical buffers, widely used in various laboratory and industrial applications:
- HEPES (4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid): A zwitterionic buffer with a pKa of 7.5, commonly used in biological and biochemical applications.
- PBS (Phosphate-Buffered Saline): A mixture of sodium phosphate and sodium chloride, commonly used in molecular biology, cell culture, and biochemistry.
- Tris (Tris(hydroxymethyl)aminomethane)): A basic buffer with a pKa of 8.3, widely used in biochemistry, molecular biology, and biotechnology applications.
- MOPS (3-(N-Morpholino)propanesulfonic acid): A zwitterionic buffer with a pKa of 7.2, commonly used in biochemistry, molecular biology, and enzymology.
- Bicine (N,N-Bis(2-hydroxyethyl)glycine): A zwitterionic buffer with a pKa of 8.3, commonly used in biochemistry, molecular biology, and biotechnology applications.
- TES (N-Tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid): A zwitterionic buffer with a pKa of 7.5, commonly used in biochemistry, molecular biology, and biotechnology applications.
- Pipes (Piperazine-N,N'-bis(2-ethanesulfonic acid)): A zwitterionic buffer with a pKa of 6.8, commonly used in biochemistry, molecular biology, and biotechnology applications.
- HCl/KCl (Hydrochloric acid/Potassium chloride): A_buffer system commonly used in analytical chemistry, pharmaceutical, and biomedical applications.
- Na2HPO4/NaH2PO4 (Sodium phosphate): A buffer system commonly used in biochemistry, molecular biology, and biotechnology applications.
- CAPS (3-Cyclohexylamino-1-propanesulfonic acid): A zwitterionic buffer with a pKa of 10.4, commonly used in biochemistry, molecular biology, and biotechnology applications.
These buffers are widely available from various manufacturers, including:
- Sigma-Aldrich (now part of Merck)
- Thermo Fisher Scientific
- Invitrogen (now part of Thermo Fisher Scientific)
- Alfa Aesar
- MP Biomedicals
- Calbiochem (now part of Merck)
When selecting a chemical buffer, consider the following factors:
- pH range: Choose a buffer with a pKa close to the desired pH.
- Buffer capacity: Select a buffer with a suitable buffer capacity for your application.
- Interference: Consider potential interference from the buffer components with your experimental system.
- Cost: Evaluate the cost of the buffer and the availability of the components.
- Stability: Choose a buffer with good chemical stability and a long shelf life.
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