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Top rated life science dyes, fluorophores and stains

Here are some top-rated life science dyes, fluorophores, and stains, categorized by their applications:

Nuclear Stains:

  1. DAPI (4',6-Diamidino-2-Phenylindole): A popular nuclear stain for fixed cells, with high sensitivity and low background fluorescence.
  2. Hoechst 33258: A cell-permeable nuclear stain for live cells, with high sensitivity and low toxicity.
  3. Propidium Iodide (PI): A membrane-impermeable nuclear stain for dead cells, often used in combination with other dyes.

Cell Membrane Stains:

  1. DiI (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate): A lipophilic dye for labeling cell membranes, with high sensitivity and photostability.
  2. DiO (3,3'-Dioctadecyloxacarbocyanine Perchlorate): A cell membrane stain with high sensitivity and low toxicity.
  3. FM 4-64 (N-(3-Triethylammoniumpropyl)-4-(6-(4-Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide): A fluorescent dye for labeling cell membranes, with high sensitivity and low toxicity.

Protein Stains:

  1. Fluorescein Isothiocyanate (FITC): A widely used fluorescent dye for labeling proteins, with high sensitivity and moderate photostability.
  2. Tetramethylrhodamine Isothiocyanate (TRITC): A fluorescent dye for labeling proteins, with high sensitivity and moderate photostability.
  3. Alexa Fluor 488: A highly photostable and sensitive fluorescent dye for labeling proteins.

RNA and DNA Stains:

  1. SYTO 9: A cell-permeable nucleic acid stain for live cells, with high sensitivity and low toxicity.
  2. SYTO 16: A cell-permeable nucleic acid stain for live cells, with high sensitivity and low toxicity.
  3. Ethidium Bromide: A nucleic acid stain for fixed cells, with high sensitivity and moderate photostability.

Mitochondrial Stains:

  1. MitoTracker Green FM: A fluorescent dye for labeling mitochondria, with high sensitivity and low toxicity.
  2. MitoTracker Red CMXRos: A fluorescent dye for labeling mitochondria, with high sensitivity and low toxicity.
  3. JC-1 (5,5',6,6'-Tetrachloro-1,1',3,3'-Tetraethylbenzimidazolylcarbocyanine Iodide): A fluorescent dye for labeling mitochondria, with high sensitivity and low toxicity.

pH and Ion Indicators:

  1. Fluorescein: A pH-sensitive fluorescent dye, with high sensitivity and moderate photostability.
  2. BCECF (2',7'-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein): A pH-sensitive fluorescent dye, with high sensitivity and moderate photostability.
  3. Fura-2: A calcium-sensitive fluorescent dye, with high sensitivity and moderate photostability.

Other Stains:

  1. Phalloidin: A fluorescent dye for labeling F-actin, with high sensitivity and low toxicity.
  2. Lysotracker Red DND-99: A fluorescent dye for labeling lysosomes, with high sensitivity and low toxicity.
  3. ER-Tracker Blue-White DPX: A fluorescent dye for labeling endoplasmic reticulum, with high sensitivity and low toxicity.