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Top rated life science dyes, fluorophores and stains
Here are some top-rated life science dyes, fluorophores, and stains, categorized by their applications:
Nuclear Stains:
- DAPI (4',6-Diamidino-2-Phenylindole): A popular nuclear stain for fixed cells, with high sensitivity and low background fluorescence.
- Hoechst 33258: A cell-permeable nuclear stain for live cells, with high sensitivity and low toxicity.
- Propidium Iodide (PI): A membrane-impermeable nuclear stain for dead cells, often used in combination with other dyes.
Cell Membrane Stains:
- DiI (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate): A lipophilic dye for labeling cell membranes, with high sensitivity and photostability.
- DiO (3,3'-Dioctadecyloxacarbocyanine Perchlorate): A cell membrane stain with high sensitivity and low toxicity.
- FM 4-64 (N-(3-Triethylammoniumpropyl)-4-(6-(4-Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide): A fluorescent dye for labeling cell membranes, with high sensitivity and low toxicity.
Protein Stains:
- Fluorescein Isothiocyanate (FITC): A widely used fluorescent dye for labeling proteins, with high sensitivity and moderate photostability.
- Tetramethylrhodamine Isothiocyanate (TRITC): A fluorescent dye for labeling proteins, with high sensitivity and moderate photostability.
- Alexa Fluor 488: A highly photostable and sensitive fluorescent dye for labeling proteins.
RNA and DNA Stains:
- SYTO 9: A cell-permeable nucleic acid stain for live cells, with high sensitivity and low toxicity.
- SYTO 16: A cell-permeable nucleic acid stain for live cells, with high sensitivity and low toxicity.
- Ethidium Bromide: A nucleic acid stain for fixed cells, with high sensitivity and moderate photostability.
Mitochondrial Stains:
- MitoTracker Green FM: A fluorescent dye for labeling mitochondria, with high sensitivity and low toxicity.
- MitoTracker Red CMXRos: A fluorescent dye for labeling mitochondria, with high sensitivity and low toxicity.
- JC-1 (5,5',6,6'-Tetrachloro-1,1',3,3'-Tetraethylbenzimidazolylcarbocyanine Iodide): A fluorescent dye for labeling mitochondria, with high sensitivity and low toxicity.
pH and Ion Indicators:
- Fluorescein: A pH-sensitive fluorescent dye, with high sensitivity and moderate photostability.
- BCECF (2',7'-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein): A pH-sensitive fluorescent dye, with high sensitivity and moderate photostability.
- Fura-2: A calcium-sensitive fluorescent dye, with high sensitivity and moderate photostability.
Other Stains:
- Phalloidin: A fluorescent dye for labeling F-actin, with high sensitivity and low toxicity.
- Lysotracker Red DND-99: A fluorescent dye for labeling lysosomes, with high sensitivity and low toxicity.
- ER-Tracker Blue-White DPX: A fluorescent dye for labeling endoplasmic reticulum, with high sensitivity and low toxicity.
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